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Preparation of MgAc 2 gradient liposomes and visual observation of the liposomes remote loaded with DOX (A) Platform illustrating the buffer exchange of MgAc 2 liposomes with 0.9% NaCl using a PD10 desalting column. (B) Photograph showing the color of MgAc 2 gradient liposomes, DOX·HCl and NaHCO 3 mixture stored at 25 °C for 5 min. (C) Photograph showing the color of the MgAc 2 gradient liposomes, DOX·HCl and NaHCO 3 mixture heated at 60 °C for 10 min.

Journal: STAR Protocols

Article Title: Protocol for preparing pH-sensitive Mg-DOX liposomes by remote loading for treatment of acidic tumors

doi: 10.1016/j.xpro.2026.104560

Figure Lengend Snippet: Preparation of MgAc 2 gradient liposomes and visual observation of the liposomes remote loaded with DOX (A) Platform illustrating the buffer exchange of MgAc 2 liposomes with 0.9% NaCl using a PD10 desalting column. (B) Photograph showing the color of MgAc 2 gradient liposomes, DOX·HCl and NaHCO 3 mixture stored at 25 °C for 5 min. (C) Photograph showing the color of the MgAc 2 gradient liposomes, DOX·HCl and NaHCO 3 mixture heated at 60 °C for 10 min.

Article Snippet: Doxorubicin Hydrochloride (DOX·HCl), USP grade , Shanghai yuanye Bio-Technology Co., Ltd. , CAS: 25316-40-9.

Techniques: Liposomes, Buffer Exchange

Characterization of Mg-DOX liposomes for particle size and Zeta potential, phospholipid concentration, and DOX concentration (A) Size distribution of the Mg-DOX liposomes measured by DLS method. (B) Cryo-TEM micrograph of the Mg-DOX liposomes. Scale bar: 100 nm. (C) Standard curve of the Stewart’s assay of DSPC concentration. (D) Standard curve of the spectrophotometric determination of DOX·HCl concentration with the microplate reader.

Journal: STAR Protocols

Article Title: Protocol for preparing pH-sensitive Mg-DOX liposomes by remote loading for treatment of acidic tumors

doi: 10.1016/j.xpro.2026.104560

Figure Lengend Snippet: Characterization of Mg-DOX liposomes for particle size and Zeta potential, phospholipid concentration, and DOX concentration (A) Size distribution of the Mg-DOX liposomes measured by DLS method. (B) Cryo-TEM micrograph of the Mg-DOX liposomes. Scale bar: 100 nm. (C) Standard curve of the Stewart’s assay of DSPC concentration. (D) Standard curve of the spectrophotometric determination of DOX·HCl concentration with the microplate reader.

Article Snippet: Doxorubicin Hydrochloride (DOX·HCl), USP grade , Shanghai yuanye Bio-Technology Co., Ltd. , CAS: 25316-40-9.

Techniques: Liposomes, Zeta Potential Analyzer, Concentration Assay